Caduceus-Hampstead University Hospital

About the RBA

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About the Robot Blood Analyzer (RBA)

The Robot Blood Analyzer is a microprocessor-based instrument that measures optical fluorescence from discreet sensors called optical electrodes (optodes). A disposable, single-use cassette contains all of the elements needed for calibration, sample measurement and waste containment. Specific calibration information from the cassette is read into the analyzer by swiping the cassette package through the bar-code reader. The cassette is then placed into the measurement chamber.

The analyzer warms the cassette to 37.0° ±0.1°C (98.6° ±0.1°F), and performs a calibration verification on the sensors for PCO2 and PO2 by passing a precision calibration gas mixture across the optode sensors. The pH and electrolyte channels are calibrated with precision buffer solution contained in the cassette. When calibration is verified, the analyzer aspirates the blood sample into the cassette and across the optode sensors. After equilibrating with the blood sample, fluorescence emission is then measured.

After a single measurement, the cassette containing the blood sample is removed from the analyzer and discarded. The analyzer contains no reagents, blood or waste.